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The mice were killed 4 h later and in vivo genotoxicity was determined by a sensitive in vivo/in vitro alkaline DNA unwinding assay for the presence of single-strand breaks and/or alkali-labile sites in hepatic DNA

To confirm the neuronal enrichment of the isolated mRNA, cDNA was used to measure the relative expression of neuron-specific MAP2 (microtubule associated protein 2) and TUBB3 (III-tubulin), microglia/macrophage-specific Iba1 (ionized calcium binding adaptor molecule 1) and CD13, astrocyte-specific GFAP (glial fibrillary acidic protein), and oligodendrocyte-specific MBP (myelin basic protein) transcripts, using the CT method

A continuous assay for monitoring the synthetic and proofreading activities of multiple aminoacyl-tRNA synthetases for high-throughput drug discovery