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doi:10.1371/journal.pone.0092573 4.Doyon S

The pre-stained biofilm was then allowed to dry for 15 min at 37C, after which it was dissolved using 80% v/v ethanol and transferred into new 96-well plate for biomass quantification at OD 550 nm using a Tecan plate reader (Infinite M1000 pro)

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Actinobacteria showed a significantly greater relative abundance in WD control mice compared to all other groups (Fig