Assessment of renal oxidative stress markers Quantification of lipid peroxidation The MDA levels were estimated by thiobarbituric acid reactive substance assay performed as demonstrated in the previous report.[] Measurement of reduced glutathione Glutathione (GSH) levels in renal tissue were assessed by Ellman's method.[] Estimation of superoxide dismutase activity The renal superoxide dismutase (SOD) levels were measured using the commercially available kit, as per the instructions provided by the manufacturer (Sigma, USA).[] Estimation of catalase activity The catalase assay was performed based on Aebi's method.[] The renal lysates were treated with hydrogen peroxide, and the absorbance of the mixture was measured
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