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During brain development there are dramatic changes in the number of synapses in the brain, with different regions of the brain developing at different rates and times (Bourgeois et al., 1994

Nat Immunol 18(5):488498 Ma C, Jiang Y, Xiang Y, Li C, Xie X, Zhang Y, You Y, Xie L, Gong J, Sun Y, Tong S, Song Q, Chen J, Xiao W (2025) Metabolic reprogramming of macrophages by biomimetic melatonin-loaded liposomes effectively attenuates acute gouty arthritis in a mouse model

The cells were incubated at 37 C, 5% CO 2 , 98% humidified incubator, and were kept sub-confluent by sub-culturing using trypsin