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The pellet containing Golgi membrane was resuspended using 1 ml of washing buffer (25 mM HEPES-KOH pH 7.4, 3M KCl, 2.5 mM Mg(OAc) 2 , 1 mg/ml soybean trypsin inhibitor), followed by incubation on ice/water for 5 minutes, and then incubated with myristoylated ARF1 (6 g/ml), ARFGAP1 (6 g/ml), BARS (3 g/ml), coatomer (6 g/ml), and GTP (2 mM) at 37C for 30 minutes in 300 l of reaction buffer (25 mM HEPES-KOH pH 7.4, 50 mM KCl, 2.5 mM Mg(OAc) 2 , 1 mg/ml soybean trypsin inhibitor, 1 mg/ml BSA, and 200 mM sucrose)

Regenerative Biology The growing field of regenerative science has increased attention toward longevity-focused peptide research

While the approach may involve surgery and medications when appropriate, the main focus is on making nutrition and lifestyle changes, as well as identifying and eradicating triggers

PMID 14207116