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At 7 d the media was aspirated, and cells were incubated at 4C with 15 ml cold PBS (Gibco, 10010-023) for 10 min

O.Gonzlez-MoralesS.Jurez-MaldonadoA.et al

38 The liver serves a crucial role in glucose homeostasis within the diabetic milieu, encompassing insulin clearance, glucose production, and the synthesis of inflammatory cytokines

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