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3.17 RBD (319-541)-His6 species separated by RP-HPLC The RP-HPLC analysis in Supplementary Figure S8 shows that the dimeric form of RBD is more hydrophobic than the monomeric form

10.1039/c9fo02487e Food Funct

Our results are in agreement with previous studies performed in bovines, where oocytes subjected to heat stress before 12 h of maturation 49 , or even in the short term at the early stage of maturation, resulted in increased Annexin-V binding and oocytes that underwent early apoptosis compared with those in the control group 51