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After collection, the tubes were stored at 80 C

The study suggests that early pollen cells may prioritize processes that are not directly useful for heat tolerance, making them more vulnerable to stress

Sample preparation (B) In an alternate protocol, the frozen liver tissue was directly lysed by probe sonication (2 10 pulse, 40% power output) in 4 M urea/DPBS and briefly cleared of debris by centrifugation (5000 g , 5 min, 4 C)