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A few hundred nanograms of the arm-selection cassette fragment was then electroporated into the electro-competent cells and positive selection was carried out at 32C on l-agar plate containing 12.5 g/ml Cam and 12.5 g/ml Kan or Amp for ~40 h

Coeliacs or other autoimmune condition sufferers Some pre-existing health conditions, including autoimmune diseases like coeliac disease, autoimmune gastritis, thyroid disease and type 1 diabetes where the body's immune system attacks its own tissues, can increase B12 deficiency risk

Grasselli G, Zanella A, Carlesso E, Florio G, Canakoglu A, Bellani G, Bottino N, et al

However, vitamin B12 injections are not without their risks