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After washing with Dulbecco's phosphate-buffered saline (DPBS), cells were incubated in DPBS containing 2 M Calcein-AM for 30 min at 37 C

Apolipoprotein E (APOE), the primary apolipoprotein and cholesterol transporter in the central nervous system (Chen et al., 2021d), drives lysosomal cholesterol sequestration in astrocytes, reducing lipid catabolism and cholesterol transport, leading to lipid dyshomeostasis and impaired A clearance (Jeong et al., 2019)

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