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427 Moreover, lung microbiome selectivity, such as cough, mucociliary clearance, and host immune defense mechanisms, is often impaired in critically ill patients as a result of both the disease itself and pharmacological interventions (e.g., sedation and corticosteroids)

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To confirm that these proteins were susceptible to glutathionylation, we treated the recombinant protein with BioGEE in vitro and analysed the extent of BioGEE labelling by Western blot using streptavidin-HRP

Additionally, conditional lethal switches or auxotrophic markers can be introduced to design the engineered bacteria to survive only in the presence of specific synthetic nutrients [206]