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Conclusions Our study provides a blueprint for systematic proactive experimental evaluation of missense variant effects on human enzymes using cell-based models, including experimental modeling of how the impact of variants depends on therapeutic context

Likewise, silibinin and epigallocatechin-3-gallate synergistically suppress lung cancer (A549) cell migration by down-regulating pro-angiogenic factors associated with the miR-1792 cluster and up-regulating anti-angiogenic factors linked to miR-19b [938]

Addressing these critical gaps demands an interdisciplinary approach integrating cryogenic electron microscopy (cryo-EM) for atomic-level structural resolution, spatially resolved crosslinking mass spectrometry to map dynamic protein interactions, and advanced metabolomic profiling to delineate real-time functional consequences of respirasome remodeling