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Semax (10 M, 0-4 h) can increase the levels of BDNF and NGF in glial cells derived from the basal forebrain of newborn rats [3]

Blots were blocked with phosphate buffered saline with 0.1% Tween 20 and then binding with antibodies for eNOS (1: 1000) or caveolin-1 (1: 1000), followed by horseradish peroxidase-conjugated goat anti-rabbit (diluted 1: 5000) and enhanced chemiluminescence reaction was performed for blots development

The aspartic acid residue is highly susceptible to dehydration, forming a cyclic imide that can then hydrolyze into either the original form or an inactive iso-aspartate analog

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