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Fourth, DN involves multiple regulated cell-death pathways with potential crosstalk, and since apoptosis-related proteins (such as the caspase family) and autophagy markers (such as LC3 and p62) were not assessed, it cannot be excluded that DAPA also modulates these pathways and contributes to the observed protective effects

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The requirement of medications as first-line treatment for these chronic conditions increases the potential cost of treatment, leaving older adults in a polypharmacy dilemma and causing many negative effects