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Positive controls, including T3 (3,3,5-Triiodo- L-thyronine sodium salt, Sigma, T6397) at 30 nM and clemastine fumarate (Sigma, SML0445) at 500 nM were used as positive controls, with their respective vehicles (DMSO and PBS) as negative controls

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When stored properly at -20C in a sealed container away from direct light and moisture, lyophilized Epithalon remains stable for 24-36 months from the date of manufacture