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For competitive transplantation, 250 freshly sorted HSCs (CD45.2) were mixed with 5 10 5 CD45.1 B6.SJL competitor bone-marrow cells and retro-orbitally injected into lethally irradiated B6.SJL recipient mice

Phase Ib/2 Study of Oral Decitabine/Cedazuridine (ASTX727) and Venetoclax in Combination with the Targeted Mutant IDH1 Inhibitor Ivosidenib or the Targeted Mutant IDH2 Inhibitor Enasidenib: 2023 Update - Oral Presentation

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The cycle can be terminated by converting gamma-glutamyl amino acids into 5-oxoproline, which subsequently turns back into glutamate