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Isopropyl -d-1-thiogalactopyranoside (IPTG) was added to a final concentration of 1 mM and the culture was further incubated at 18 C overnight

Liu B, Lou Q, Smith H, Velez-Cortes F, Dillmann WH, Knollmann BC et al (2020) Conditional up-regulation of SERCA2a exacerbates RyR2-dependent ventricular and atrial arrhythmias

Declining renal function leads to an accumulation of circulating uremic toxins that may lead to that may lead to local and systemic inflammation and induce profibrotic effects [56,57,58]

For example, chromosomal instability activates STING-dependent non-canonical NF-B signaling, promoting epithelial-mesenchymal transition, cell invasion, and metastasis (110)