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Immunostaining of the fat pad for UCP1 revealed robust browning of white adipose tissue, which on day 3 was faster and more pronounced than previously reported after 4 weeks of CR 34 (Fig

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This suggests that long-term maintenance on an LD rich in carbohydrates may confound some of the microbiome differences commonly reported between mice on HFDs and those on lean control diets which contain fewer carbohydrates