alpha lipoic acid precursor glutathione Benefits, Uses and Best Practices Frontiers | Diabetes and Alpha

Protein concentrations were determined using a DS-11 spectrophotometer (DeNovix)

Nelson et al., 2012

Companion satellite-cell cultures, prepared at the same time, were immunostained at 30-h post-plating with D3 anti-desmin mAb and DAB (a chromogenic HRP-substrate that forms an insoluble brown reaction product) to determine the percentage of myogenic cells present 18 ,19 cultures with less than 95% DAB-positive cells (namely, less than 95% desmin-positive myogenic purity) were not used for experiments just to ensure the reliability of data specific to myogenic cells in the employed primary cell culture systems

& Cummings, J