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Bibcode:2005BCB....83..354M

Bindels LB, Beck R, Schakman O, Martin JC, de Backer F, Sohet FM, Dewulf EM, Pachikian BD, Neyrinck AM, Thissen JP, Verrax J, Calderon PB, Pot B, Grangette C, Cani PD, Scott KP, Delzenne NM

Briefly, different concentrations of TLC-2716 or TLC-6665 were incubated with an assay buffer (Tris/HCl buffer (pH 6.8) containing 240 mM KCl, 1 g l 1 bovine serum albumin and 0.002% Triton X-100) containing 530 g ml 1 recombinant GST-tagged LXR/LXR LBD, 125 pg l 1 GST-Tb cryptate (CisBio), 400 nM N-terminally biotinylated NCOA3 (coactivator) peptide, 1 M 24( S )-25-epoxycholesterol (LXR agonist, only in the NCOA3 assay) and 2.5 ng l 1 Streptavidin-XL665 (CisBio) for 1 h at 4 C, following which fluorescence was measured in a VictorX4 multiplate reader (PerkinElmer Life Science) using 340 nm as excitation and 615 and 665 nm as emission wavelengths

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