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Figure 7 Discussion AX is a widely used antibiotic and one of the most frequently involved in allergic reactions

*Correspondence: Xiangrong Song, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

So do environmental toxins like pesticides, heavy metals, and air pollutants

First, we sought to identify a medium that would support assembloid formation, that is, the culture of all three cell types: hepatocytes, cholangiocytes/ductal cells and portal mesenchyme without overgrowth of any of them, we tested several media and found that a minor adaptation of the DM medium used for h-HepOrgs differentiation without A8301 (assembloid medium) supported culture of the three cell types while preventing their overgrowth