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Finally, they were resuspended in 1 mL of 100 mM Tris solution and the total proteins present in the final samples were quantified using QuantiPro BCA assay (Sigma Aldrich)

This enables the development of personalized dosing regimens or adjunctive microecological interventions (e.g., probiotic/prebiotic supplementation) to enhance therapeutic efficacy while reducing the incidence of drug resistance and adverse effects

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