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West Pico ECL (#34577) was purchased from Pierce

coli cells were washed with the PBS buffer for three times at 4C, and suspended in the 5 ml PBS solution, respectively

Blood-brain barrierpenetrating single CRISPR-Cas9 nanocapsules for effective and safe glioblastoma gene therapy | Science Advances

MMP2 and MMP9 degrade collagen and other matrix proteins to allow trophoblast penetration, while TIMP1 and TIMP2 limit tissue damage during this process