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After washing with PBS for 3 times, cells were permeabilized with 0.1% Triton X-100 for 10 min and blocked with 5% normal goat serum with 0.01% Triton X-100 in PBS for 1 hr at room temperature

(Reference ODoherty, Freedman and Hollenbeck62) These findings highlight the impact of not only excess weight, as assessed through BMI, but also body fat distribution on GERD (Reference Krner, Corts and Lukens59)

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Russell (Gillig et al., 2000) demonstrated that IM-MS can be used to analyze the structures of peptides and proteins, as well as to study changes in the collision cross-section (CCS) of a protein (which is related to its size and shape) associated with the changes in its charge state