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Materials and methods Strains and culture conditions Yeasts were maintained and grown either in YPD broth [1% yeast extract (Oxoid), 2% bacteriological peptone (Oxoid), 2% D-glucose], or in YNB broth [0.69% yeast nitrogen base without amino acids (Formedium), 2% D-glucose] supplemented as required with amino acids, uracil, 5-FOA (Melford), D-histidine (Sigma) or reduced GSH (Sigma)

As a control, normal mice (without tumor) were injected with DOX&IR780@PEG-PCL-SS NPs and imaged at 6, 12, 24 and 48 h after injection using the in vivo imaging system