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After the experimental treatments, the cell supernatant in the 96-well plate was discarded, 10 L MTT solution and 90 l culture solution were added to the pre-designed cell wells

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Therefore, it should be noted that PFK-1 functionally couples glycolysis to the citric acid cycle

Eur J Appl Physiol Occup Physiol 56:639642 Furuichi Y, Goto-Inoue N, Manabe Y, Setou M, Masuda K, Fujii NL (2014) Imaging mass spectrometry reveals fiber-specific distribution of acetylcarnitine and contraction-induced carnitine dynamics in rat skeletal muscles