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some of these cells create brainbody barriers (perineurial and subperineurial surface glial cells), others cover neuronal somata (cortex glia) and some ensheath synapses in neuropil (astroglia-like cells) 32,33

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Protein lysates were then cleared in a refrigerated table-top microcentrifuge (Eppendorf) at 21,100 g for 10 min