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Ferroptosis inducers such as erastin or RSL3 have been shown to enhance the anticancer effects of cisplatin during the treatment of various cells from lung, colorectal, ovarian, and pancreatic ductal adenocarcinoma cancers through inhibition of system Xc- or GPX4 [66]

Molecular dynamics and structure-activity relationships identify molecular determinants of isoxazole 1 inhibition of Cn Acs1 To further characterize the interactions that contribute to the binding of isoxazole 1 to Cn Acs1, we performed molecular dynamics (MD) simulations using Schrdinger software as described in the Methods and Table S4

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Extracts were cleared by centrifugation at 15,000 g for 15 min at 4 C, and supernatants were incubated with 2 g of purified GST-tagged GFP-Nanobody for 30 min on ice