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A metabolome analysis disclosed that sulphasalazine triggers an increase in the glutamate-derived TCA cycle intermediate -ketoglutarate in addition to a decrease in cysteine and glutathione

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For example, if the glutaredoxin sulfur atom in Grx(SSR) species was accessible for a productive reaction with GSH, one would not expect to trap such species by monothiol glutaredoxin mutants 83,84,85