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Kozlowski MT, Crook CJ, Ku HT (2021) Towards organoid culture without matrigel

Therefore, this metabolic microenvironment of solid tumors can be recapitulated, and throughout the study we used 0.5 mM glucose in media to recapitulate glucose deprivation and, on top of this, added 20 mM lactic acid for a mimicry of lactic acidosis