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doi:10.1186/s12883-024-04007-1 7

W., Benjamin, I

For WB, 30 g of proteins from tissue and cell lysates were mixed with 8 L of sample buffer (Tris 0.5% (pH 6.8), glycerol 20%, sodium dodecylsulfate (SDS) 10%, 2--mercaptoethanol, bromophenol blue 0.1%), and RIPA buffer to a total sample volume of 32 L