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[114] PacBio SMRT sequencing offers methylation detection as part of PCR-free DNA sequencing Oxford Nanopore Technologies DNA Sequencing can also be done with simultaneous Methylation calling if the DNA was not amplified by PCR prior to sequencing Differentially methylated regions (DMRs) [edit] Differentially methylated regions, which are genomic regions with different methylation statuses among multiple samples (tissues, cells, individuals or others), are regarded as possible functional regions involved in gene transcriptional regulation