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Analysis of the metabolic flux For the metabolic flux analysis, L-glutamine ( 13 C 5 , 99%) was procured from Cambridge Isotope Laboratories (MA, USA)

Gene ontology analysis of proteins altered by BSO treatment (bottom)

7d), confirming that the need to increase mitochondrial mass in 5FU-treated cells is driven by a dependence on increased mitochondrial activity, and metformin can block this mitochondrial adaptation to prevent drug tolerance in tumours