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freezing causes ice crystal formation that ruptures peptide bonds irreversibly

After washing five times in lysis buffer, the beads were resuspended in SDS-containing gel sample buffer and electrophoresed on an SDSpolyacrylamide gel electrophoresis (PAGE) gel

[DOI] [PMC free article] [PubMed] [Google Scholar] 55.Perrin R., Brianon S., Jeandel C., Artur Y., Minn A., Penin F., Siest G

For example, the optimal pH for the peroxidase-like activity of nanozymes such as Fe 3 O 4 28 , Pt-Ft 147 , and irregular-shaped Pt 164 , and for the oxidase-like activities of nanozymes such as Cu-MOF 165 , N-doped carbon dots 166 , and Pd@Ir 167 typically ranges from 3 to 5