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cDNA libraries were constructed by random priming using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) and then used as a template for qPCR amplification with Luna Universal qPCR Master Mixes (New England Biolabs) on an AriaMx cycler (Agilent)

That trial was published in Obstetrics and Gynecology (2019)

With inputs of experimental isotopologue patterns for metabolite substrates, we then calculated the predicted isotopologue patterns of -Glu-Ser-Gly in the scenarios of two different reaction pathways using the joint probability (Fig

It was developed by Metabolic Pharmaceuticals, an Australian biopharmaceutical company, in the late 1990s as a potential therapeutic for obesity and metabolic disorders