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Damage-associated molecular patterns (DAMPs) released by injured neurons, such as pathological proteins, extracellular ATP, and high mobility group box 1 protein (HMGB1), can activate pattern recognition receptors on the glial cell surface, triggering neuroinflammation ( In conclusion, neuroinflammation serves as a critical bridge connecting peripheral pathology and central symptoms in MG, while age-related factors significantly influence MG onset and progression through immunosenescence, neuromuscular junction degeneration, and activation of the neuroinflammation-oxidative stress-mitochondrial dysfunction axis

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Each sample was then split into two fraction (100 L) and treated as follows: For H 2 S measurement, 100 L of each sample was precipitated with 1 mL of absolute MeOH at 40 C for 2 h, centrifuged (16,000 g at 0 C), and obtained supernatant was evaporated and the residue was resuspended and sonicated in 100 L of 50 mM HEPES buffer (pH 7.4)

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