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10 l of the extracts were added to 90 l of reaction buffer containing 200 mM bicine, pH 8.0, 8 mM EDTA, 3.2 mM phenazine methosulfate, 0.84 mM 3-(4,5-dimethylthiazol- 2-yl)2,5- diphenyltetrazolium bromide, 20% ethanol and 0.4 g alcohol dehydrogenase

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To conclude, the endocannabinoid system in the human body plays a key role in a multitude of physiological functions including inflammatory and immunological responses, pain, temperature control, endocrine regulation, feeding behaviour, learning and memory, emotional processing, and sleep

Then all of a sudden, I experienced lower back pain maybe because I have a weak kidneys since 2013 and was also hospitalized because of kidney infection