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doi: 10.3390/molecules26102941 456 ZhangYGuJWangYZhaoZWangZLiW

G.StevensJ

Teng MW, Swann JB, Koebel CM, Schreiber RD, Smyth MJ

EMSA EMSAs were performed by mixing increasing amounts of wt or mutated BRD4 ET (with or without ASXL1 peptide at a 1:1 molar ratio) with 10 nM/lane of 147 bp 601 DNA in 2025 mM Tris pH 7.5, 150 mM NaCl and 5 mM DTT in a 10 L reaction volume