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GST-tagged proteins are bound to the affinity resin, and impurities are removed by washing with binding buffer GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione

doi: 10.1097/01.CCM.0000085090.06078.8C

In the nucleus, following heterodimer complex formation with transcription factors, like small Maf proteins (G/F/K) and c-Jun, Nrf2 complexes bind to the antioxidant response element (ARE), a regulatory enhancer region within gene promoters that upregulate antioxidant and anti-inflammatory defense processes (Cuadrado et al., 2019

In a study on pancreatic cancer radiotherapy, MDSC immunosuppressive phenotypes were enhanced by lactate via the GPR81/mTOR/HIF-1/STAT3 pathway (128) ( Figure 2 )