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This is consistent with the previously reported titration of GSSG during the reductive half-reaction of the U597C enzyme for which minimal kinetic changes were detected (Smith et al., 2023), suggesting that all phases observed in the transient state occur prior, are not contingent on, and are more rapid than the reduction of the C28-C31 pair or GSSG

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Additionally, castration considerably decreased the expression of important antioxidant enzymes (AOEs) (GPx1, thioredoxin, SOD2, and peroxiredoxin 5) and markedly increased the expression of ROS-generating NAD(P)H oxidases

This leads to both the direct nitration and inactivation of the TCR as well as nitration and inhibition of the chemokines CCL2 and CCL5, leading to decreased T cell accumulation within tumors (8486)