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Extracellularly, pancreatic -cells release hepcidin, which induces the internalization of binding transferrin [123124]

According to the excitation/emission wavelengths (488 nm/522 nm) of the DCFH-DA probes in the manufacturers instructions, appropriate channels were selected to measure the fluorescence values, and the data were analyzed using Flowjo V10 software (FlowJo LLC, USA)