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doi:10.4103/0019-5049.60498

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The protein was extracted from cells by resuspending the pellet with 20 mM HEPES pH 8.0, 150 mM NaCl, 2 g ml 1 aprotinin, 2 g ml 1 pepstatin, 2 g ml 1 leupeptin, 1 mM phenylmethanesulfonyl fluoride (PMSF), and solubilized with 1% (w/v) n-dodecyl--D-maltoside (DDM