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Cellular senescence was assessed by SA--galactosidase staining (SA--gal staining) using senescence -galactosidase Staining Kit (Cell Signaling Technology, Beverly, MA, United States) according to the manufacturers instructions

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was in the midst of a Public Health Emergency (PHE) that incurred millions of cases and over 718,000 COVID-19 deaths (86 FR 63825 and 86 FR 63875 through 63876, respectively)