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To confirm the DQ8 transgenic phenotype, 510 5 mesenteric lymph-node lymphocytes were stained extracellularly as reported (Ruiz-Iglesias et al, 2022), with the fluorochrome-conjugated anti-mouse mAbs anti-HLA-DR/DP/DQ-FITC (BD Bioscience), anti-MHC-II-Alexa-Fluor-700, and anti-CD45-PE (both TFS), and assessed by flow cytometry analysis using an Aurora cytometer (Cytek Biosciences) at the Flow Cytometry Unit of the Scientific and Technological Centres of the University of Barcelona (CCiTUB