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The protein splicing is a process in which the intervening sequence (intein) removes itself and binds the flaking residues (exteins) to produce two independent protein products (Perler et al., 1994)

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The results indicated that scaffold implantation helps maintain intervertebral disc height, reduces NP moisture loss, and partially restores the biomechanical function of the IVD ( In vitro and in vivo experiments demonstrated that this composite scaffold enhanced osteogenic differentiation, promoted angiogenesis, and accelerated abnormal NP degeneration, making it suitable for lumbar interbody fusion (Zhang et al., 2025b)