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What tests are you taking and how are you assessing on day one

ZFO NPs (final concentration 100 g mL 1 ) were co-incubated with H 2 O 2 (final concentration 0.5 mM) and TMB (final concentration 250 g mL 1 ) for 30 min, and the absorption curve of ZFO + H 2 O 2 + TMB group was recorded by UV-vis ultraviolet spectrophotometer

Redox thresholds determine the responsiveness of the system, as the release is triggered when intracellular glutathione (GSH) levels surpass a specific threshold, typically elevated in cancer cells