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Aliquots were centrifuged at 13,000 rpm for 10 min at 4 C, and 150 L supernatant was transferred to Chromacol vials containing 100 L water for dilution

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Although a number of creative and successful approaches in animal models have been employed to deliver growth factors like GDNF (Wang et al., 2011) to the brain, these methodologies are technically complex and prohibitively difficult to bring to practice with large numbers of patients

Understanding this mechanism has opened new opportunities to modulate inflammation and protect neurons in disorders like AD, PD, and HD