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Competing interests None of the authors have any conflict of interests

W.GriersonD

Following a 1 h incubation with the appropriate secondary antibody, Clarity Western ECL Substrate was applied to the membrane and bands were visualized using a Biorad Chemidoc MP to first pixel saturation and densitometry performed using Image Lab (Biorad, Hercules, CA)

The authors showed that administration of leucine or leucine-enriched proteins (at the doses of 1.26 g leucine/day) is well-tolerated and significantly improves sarcopenia in older individuals, mainly by improving lean muscle-mass content