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The higher concentrations reduced metabolic activity oxidative phosphorylation, measured using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide, MTT and caused significant leakage of lactate dehydrogenase (LDH) enzymatic activity into the tissue culture medium

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TSC2 Integrates Wnt and Energy Signals via a Coordinated Phosphorylation by AMPK and GSK3 to Regulate Cell Growth