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Description

In their experimental paradigm, mCherry-labeled MDA-MB-231 cells were microinjected into the PVS or Cuvier ducts of Casper (roy -/- ;nacre -/- ) embryos with ubiquitous enhanced green fluorescent protein (EGFP) expression in vasculature, allowing real-time tracking of metastatic cell behavior and tumor-induced vasculogenesis via confocal time-lapse imaging

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Starter culture was used to inoculate fresh LB media which was grown then induced using iso -propyl--d-1-thiogalactopyranoside (IPTG)

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